|
| 1. |
Turci, M., Sardaro, M.L.S., Visioli, G., Maestri, E., Marmiroli, M. and Marmiroli, N.
(2009)
Evaluation of DNA extraction procedures for traceability of various tomato products.
Food Control
[epub ahead of print]
,
.
|
| |
Notes:
In this study, the authors wanted to examine the ability to trace the origin of tomato goods from fresh to processed. They tested several DNA extraction procedures for fresh tomato, tomato sauce, tomato puree, tomato pulp, whole peeled S. Marzano PDO (Protected Designation of Origin) tomato, whole peeled tomato, tomato concentrate and ‘‘Arrabbiata sauce”. Homogenized material (200mg) was extracted in three replicates using seven different methods including the Wizard® DNA Clean-Up System. The DNA extracted was then analyzed by agarose gel electrophoresis, quantified and tested in PCR using SSR loci. The authors concluded that the Wizard® DNA Clean-Up System was the most effective of the DNA extraction methods tested and yielded the greatest number of successful amplification reactions with lowest investment of personnel time and money.
(0004003) |
| |
 |
| |
Products: Wizard® DNA Clean-Up Resin | Wizard® DNA Clean-Up System |
| 2. |
Wang, P.Y., Neretti, N., Whitaker, R., Hosier, S., Chang, C., Lu, D., Rogina, B., and Helfand, S.L.
(2009)
Long-lived Indy and calorie restriction interact to extend life span.
Proc. Natl. Acad. Sci. U S A
106(23)
,
9262-7
.
|
| |
Notes:
These authors investigated the relationship between calorie restriction and INDY expression on lifespan in Drosophila melanogaster. They showed that calorie restriction downregulates INDY expression in normal flies. INDY mutants on a normal diet had increased lifespan that was not extended further by calorie restriction. The INDY long-lived flies also shared several phenotypic characteristics with normal flies on a calorie-restricted diet. The authors then demonstrated that the phenotypic effects of the INDY mutation were not caused by reduced ability to intake food. The results show that INDY and calorie restriction interact to extend lifespan, and that decreased INDY expression induces a calorie-restriction-like status. During the study, the Maxwell 16 Tissue DNA Purification System was used to isolate DNA from Drosophila. The DNA was used in PCR analyses to confirm the absence of Wolbachia DNA in the experimental lines.
(0004001) |
| |
|
| |
Products: Maxwell® 16 Instrument | Maxwell® 16 Tissue LEV Total RNA Purification Kit |
| 3. |
Cheung, Q.C., Turner, P.V., Song, C., Wu, D., Cai, H.Y., MacInnes, J.I. and Li, J.
(2008)
Enhanced resistance to bacterial infection in protegrin-1 transgenic mice.
Antimicrob. Agents Chemother.
52
,
1812–9
.
|
| |
Notes:
One potential source of antibiotic-resistant bacteria is food-producing animals. The authors examined the ability of protegrin-1 (PG-1), an antimicrobial peptide, to protect wildtype and transgenic mice expressing PG-1 against bacterial infection. As part of the cloning strategy to produce the PG-1 expression construct, the authors amplified and cloned full-length PG-1 into the pGEM®-T Easy Vector. To test the bactericidal activity of PG-1 expressed in transgenic mice, radial diffusion assays were performed, in which test samples were added to a well containing E. coli and the clear antibacterial zone was measured. Two of the test samples were neutrophil secretions from the PG-1 transgenic mice and purified polyhistidine-tagged PG-1 protein, purified using the MagneHis™ Protein Purification System.
(0003896) |
| |
|
| |
Products: MagneHis™ Protein Purification System | pGEM®-T Easy Vector System I |
| 4. |
Nakayama, A., Okayama, A., Hashida, M., Yamamoto, Y., Takebe, H., Ohnaka, T., Tanaka, T. and Imai, S.
(2006)
Development of a routine laboratory direct detection system of staphylococcal enterotoxin genes.
J. Med. Microbiol.
55
,
273–277
.
|
| |
Notes:
In this study, a real-time PCR assay coupled with a DNA extraction method were used to detect staphylococcal enterotoxin (SE)-encoding genes in milk, a source of staphylococcal food poisoning. Pasteurized milk prepared with known concentrations of Staphylococcus aureus was used to generate a standard curve; experimental samples were from a staphylococcal food-poisoning outbreak that occurred in Japan in June 2000. PCR inhibition was overcome by using the following DNA purification method: a sample of milk (100µl) was added to an equal volume of 0.2M sodium hydroxide and incubated at 37°C for 20 minutes. The alkaline-treated sample was neutralized using 10µl of 3M sodium acetate (pH 5.4), extracted with 1ml of petroleum ether, and centrifuged at 13,000 × g for 10 minutes at 25°C. The aqueous phase was transferred to a fresh tube and bacterial DNA was purified from the aqueous solution using the Wizard® SV Genomic DNA Purification System.
(0003677) |
| |
|
| |
Products: Wizard® SV 96 Genomic DNA Purif. Start-Up Kit, 110V Electrical (4 x 96 preps, manifold & free vacuum pump) | Wizard® SV 96 Genomic DNA Purif. Start-Up Kit, 220V Electrical (4 x 96 preps, manifold & free vacuum pump) | Wizard® SV 96 Genomic DNA Purif. Start-Up Kit, 230V Electrical (4 x 96 preps, manifold & free vacuum pump) | Wizard® SV 96 Genomic DNA Purification System |
| 5. |
Fumière, O., Dubois, M., Baeten, V., von Holst, C. and Berben, G.
(2006)
Effective PCR detection of animal species in highly processed animal byproducts and compound feeds.
Anal. Bioanal. Chem.
385
,
1045-1054
.
|
| |
Notes:
The authors developed a PCR method to detect the presence of meat and bone meal (MBM) in animal feed even if the MBM had been heat treated, and discern whether the animal component is bovine or porcine in origin. The genomic DNA from 100mg of various feedstuffs with known and unknown amounts of MBM, fishmeal or poultry feed or a combination of these compounds was isolated using the Wizard® Magnetic DNA Purification System for Food with the KingFisher System. Real-time PCR was performed using 5μl of extracted DNA.
(0003750) |
| |
|
| |
Products: Wizard® Magnetic DNA Purification System for Food |
| 6. |
Weller, S.A., Stead, D.E. and Young, J.P.W.
(2004)
Acquisition of an Agrobacterium Ri plasmid and pathogenicity by other α-Proteobacteria in cucumber and tomato crops affected by root mat.
Appl. Environ. Microbiol.
70
,
2779–2785
.
|
| |
Notes:
Researchers used the Wizard® Genomic DNA Purification Kit to isolate genomic DNA from non-Agrobacterium field bacteria samples. The isolated genomic DNA was used in PCR to amplify regions of the 16S rRNA gene. The PCR products were cleaned up using the Wizard® SV Gel and PCR Clean-Up System and used in sequencing reactions. The paper also mentions the use of the Wizard® Magnetic DNA Purification System for Food to isolate Agrobacterium radiobacter from cucumber root mats grown in the lab.
(0003190) |
| |
|
| |
Products: Wizard® Genomic DNA Purification Kit | Wizard® Genomic DNA Purification Kit | Wizard® Magnetic DNA Purification System for Food | Wizard® SV Gel and PCR Clean-Up System |
| 7. |
Bailey, A.M., Pajak, L., Fruchey, I.R., Cowan, C.A. and Emanuel, P.A.
(2003)
Robotic nucleic acid isolation using a magnetic bead resin and an automated liquid handler for biological agent simulants.
J. Assoc. Lab. Automation
8(6)
,
113-120
.
|
| |
Notes:
This study describes the development of a system that can rapidly and accurately detect traces of biological agents from environmental samples. Using Erwinia herbicola and Bacillus subtilis var. niger as models for potential biological warfare agents, a method for DNA extraction using the Wizard® Magnetic DNA Purification System for Food, MagneSil® Blood Genomic, Max Yield System and a combination of the two was automated on a Beckman Coulter Biomek® FX robotic liquid handling system. The isolated DNA was used in a Taqman® real-time PCR assay that specifically amplified and identified DNA species. The ability of the MagneSil®-based DNA purification technology to eliminate PCR inhibitors was also evaluated. Various soil samples, surface swabs and air samples were mixed with bacterial cultures to see if any contaminants present in the samples inhibited PCR. It was found that the modified MagneSil® method described here eliminated many PCR inhibitors.
(0003094) |
| |
|
| |
Products: MagneSil® Blood Genomic, Max Yield System | Wizard® Magnetic DNA Purification System for Food |